[HTML][HTML] Non-classical tissue monocytes and two functionally distinct populations of interstitial macrophages populate the mouse lung
J Schyns, Q Bai, C Ruscitti, C Radermecker… - Nature …, 2019 - nature.com
Nature communications, 2019•nature.com
Resident tissue macrophages (RTM) can fulfill various tasks during development,
homeostasis, inflammation and repair. In the lung, non-alveolar RTM, called interstitial
macrophages (IM), importantly contribute to tissue homeostasis but remain little
characterized. Here we show, using single-cell RNA-sequencing (scRNA-seq), two
phenotypically distinct subpopulations of long-lived monocyte-derived IM, ie CD206+ and
CD206− IM, as well as a discrete population of extravasating CD64+ CD16. 2+ monocytes …
homeostasis, inflammation and repair. In the lung, non-alveolar RTM, called interstitial
macrophages (IM), importantly contribute to tissue homeostasis but remain little
characterized. Here we show, using single-cell RNA-sequencing (scRNA-seq), two
phenotypically distinct subpopulations of long-lived monocyte-derived IM, ie CD206+ and
CD206− IM, as well as a discrete population of extravasating CD64+ CD16. 2+ monocytes …
Abstract
Resident tissue macrophages (RTM) can fulfill various tasks during development, homeostasis, inflammation and repair. In the lung, non-alveolar RTM, called interstitial macrophages (IM), importantly contribute to tissue homeostasis but remain little characterized. Here we show, using single-cell RNA-sequencing (scRNA-seq), two phenotypically distinct subpopulations of long-lived monocyte-derived IM, i.e. CD206+ and CD206−IM, as well as a discrete population of extravasating CD64+CD16.2+ monocytes. CD206+ IM are peribronchial self-maintaining RTM that constitutively produce high levels of chemokines and immunosuppressive cytokines. Conversely, CD206−IM preferentially populate the alveolar interstitium and exhibit features of antigen-presenting cells. In addition, our data support that CD64+CD16.2+ monocytes arise from intravascular Ly-6Clo patrolling monocytes that enter the tissue at steady-state to become putative precursors of CD206−IM. This study expands our knowledge about the complexity of lung IM and reveals an ontogenic pathway for one IM subset, an important step for elaborating future macrophage-targeted therapies.
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